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JCS ePress online publication date 8 Apr 2008
doi: 10.1242/jcs.017665

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Research Article

Dynamics of an F-actin aggresome generated by the actin-stabilizing toxin jasplakinolide


Francisco Lázaro-Diéguez, Carmen Aguado, Eugenia Mato, Yován Sánchez-Ruíz, Inmaculada Esteban, Jordi Alberch, Erwin Knecht, and Gustavo Egea*
* Author for correspondence (e-mail: gegea{at}ub.edu)

In this study, we report the formation of several cytoplasmic inclusion bodies composed of filamentous actin (F-actin) and generated by experimental treatments using depolymerizing or stabilizing actin toxins in neuronal and non-neuronal mammalian cell lines. The actin-stabilizing toxin jasplakinolide (Jpk) induced, in a microtubule-dependent manner, a single, large F-actin aggregate, which contained {beta}- and {gamma}-actin, ADF/cofilin, cortactin, and the actin nucleator Arp2/3. This aggregate was tightly associated with the Golgi complex and mitochondria, and was surrounded by vimentin intermediate filaments, microtubules and MAP4. Therefore, the Jpk-induced single, large F-actin aggregate fits the established criteria for being considered an aggresome. Lysosomes and/or autophagic vacuoles, proteasomes and microtubules were found to directly participate in the dissolution of this F-actin aggresome. Finally, the model reported here is simple, highly reproducible and reversible, and it provides an opportunity to test pharmacological agents that interfere with the formation, maintenance and/or disappearance of F-actin-enriched pathological inclusion bodies.






© The Company of Biologists Ltd 2008