Freeze-fracture replica electron microscopy combined with SDS digestion for cytochemical labeling of integral membrane proteins. Application to the immunogold labeling of intercellular junctional complexes

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Andersson-Forsman, C. and Pinto da Silva, P (1988). Fracture-flip: new high-resolution images of cell surfaces after carbon stabilization of freeze-fractured membranes. J. Cell Sci 90, 531-541.[Abstract/Free Full Text]

Andersson-Forsman, C. and Pinto da Silva, P (1988). Label-fracture of cell surfaces by replica staining. J. Histochem. Cytochem 36, 1413-1418.[Abstract]

Dinchuk, J. E., Johnson, T. J. A. and Rash, J. E (1987). Postreplication labeling of E-leaflet molecules: membrane immunoglobulins localized in sectioned, labeled replicas examined by TEM and HVEM. J. Electron Microsc. Tech 7, 1-16.[Medline]

Fujimoto, K. and Pinto da Silva, P (1988). Macromolecular dynamics of the cell surface during the formation of coated pits is revealed by fracture-flip. J. Cell Sci 91, 161-173.[Abstract/Free Full Text]

Fujimoto, K. and Pinto da Silva, P (1989). Surface views of nuclear pores in isolated rat liver nuclei as revealed by fracture-flip/Triton-X. Eur. J. Cell Biol 50, 390-397.[Medline]

Furuse, M., Hirase, T., Itoh, M., Nagafuchi, A., Yonemura, S., Tsukita, Sa. and Tsukita, Sh (1993). Occludin: a novel integral membrane protein localizing at tight junctions. J. Cell Biol 123, 1777-1788.[Abstract/Free Full Text]

Goodenough, D. A. and Gilula, N. B (1974). The splitting of hepatocyte gap junctions and zonulae occlusentes with hypertonic disaccharides. J. Cell Biol 61, 575-590.[Abstract/Free Full Text]

Gruijters, W. T. M., Kistler, J., Bullivant, S. and Goodenough, D. A (1987). Immunolocalization of MP70 in lens fiber 16-17 nm intercellular junctions. J. Cell Biol 104, 565-572.[Abstract/Free Full Text]

Heuser, J. E., Reese, T. S., Jan, L. Y., Dennis, M. J. and Evans, L (1979). Synaptic vesicle exocytosis captured by quick-freezing and correlated with quantal transmitter release. J. Cell Biol 81, 275-300.[Abstract/Free Full Text]

Koch, P. J., Walsh, M. J., Schmelz, M., Goldscmidt, M. D., Zimbelmann, R. and Franke, W. W (1990). Identification of desmoglein, a constitutive desmosomal glycoprotein, as a member of the cadherin family of cell adhesion molecules. Eur. J. Cell Biol 53, 1-12.[Medline]

Kuraoka, A., Iida, H., Hatae, T., Shibata, Y., Itoh, M. and Kurita, T (1993). Localization of gap junction proteins, connexins 32 and 26, in rat and guinea pig liver as revealed by quick-freeze, deep-ech immunoelectron microscopy. J. Histochem. Cytochem 41, 971-980.[Abstract]

Pinto da Silva, P. and Branton, D (1970). Membrane splitting in freeze-eching. Covalently labelled ferritin as a membrane marker. J. Cell Biol 45, 598-605.[Abstract/Free Full Text]

Pinto da Silva, P. and Kachar, B (1982). On tight-junction structure. Cell 28, 441-450.[Medline]

Pinto da Silva, P. and Kan, F. W (1984). Label-fracture: a method for high resolution labeling of cell sufaces. J. Cell Biol 99, 1156-1161.[Abstract/Free Full Text]

Takeda, A., Kanoh, M., Shimazu, T. and Takeuchi, N (1988). Monoclonal antibodies recognizing different epitopes of the 27-kDa gap junction protein from rat liver. J. Biochem 104, 901-907.

Yoshida-Noro, C., Suzuki, N. and Takeichi, M (1984). Molecular nature of the calcium-dependent cell-cell adhesion system in mouse teratocarcinoma and embryonic cells studied with a monoclonal antibody. Dev. Biol 101, 19-27.[Medline]

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				H. Robenek, O. Hofnagel, I. Buers, S. Lorkowski, M. Schnoor, M. J. Robenek, H. Heid, D. Troyer, and N. J. Severs Butyrophilin controls milk fat globule secretion PNAS, July 5, 2006; 103(27): 10385 - 10390. [Abstract] [Full Text] [PDF]

				M. Osumi, M. Konomi, T. Sugawara, T. Takagi, and M. Baba High-pressure freezing is a powerful tool for visualization of Schizosaccharomyces pombe cells: ultra-low temperature and low-voltage scanning electron microscopy and immunoelectron microscopy J. Electron Microsc. (Tokyo), April 1, 2006; 55(2): 75 - 88. [Abstract] [Full Text] [PDF]

				W. Sheng, G. Wang, D. P. La Pierre, J. Wen, Z. Deng, C.-K. A. Wong, D. Y. Lee, and B. B. Yang Versican Mediates Mesenchymal-Epithelial Transition Mol. Biol. Cell, April 1, 2006; 17(4): 2009 - 2020. [Abstract] [Full Text] [PDF]

				J. Ikenouchi, M. Furuse, K. Furuse, H. Sasaki, S. Tsukita, and S. Tsukita Tricellulin constitutes a novel barrier at tricellular contacts of epithelial cells J. Cell Biol., December 19, 2005; 171(6): 939 - 945. [Abstract] [Full Text] [PDF]

				M. Koike, M. Shibata, S. Waguri, K. Yoshimura, I. Tanida, E. Kominami, T. Gotow, C. Peters, K. von Figura, N. Mizushima, et al. Participation of Autophagy in Storage of Lysosomes in Neurons from Mouse Models of Neuronal Ceroid-Lipofuscinoses (Batten Disease) Am. J. Pathol., December 1, 2005; 167(6): 1713 - 1728. [Abstract] [Full Text] [PDF]

				G. A. Zampighi, A. M. Planells, D. Lin, and D. Takemoto Regulation of Lens Cell-to-Cell Communication by Activation of PKC{gamma} and Disassembly of Cx50 Channels Invest. Ophthalmol. Vis. Sci., September 1, 2005; 46(9): 3247 - 3255. [Abstract] [Full Text] [PDF]

				H. Robenek, M. J. Robenek, I. Buers, S. Lorkowski, O. Hofnagel, D. Troyer, and N. J. Severs Lipid Droplets Gain PAT Family Proteins by Interaction with Specialized Plasma Membrane Domains J. Biol. Chem., July 15, 2005; 280(28): 26330 - 26338. [Abstract] [Full Text] [PDF]

				H. Robenek, M. J. Robenek, and D. Troyer PAT family proteins pervade lipid droplet cores J. Lipid Res., June 1, 2005; 46(6): 1331 - 1338. [Abstract] [Full Text] [PDF]

				X. Guo, J. N. Rao, L. Liu, T. Zou, K. M. Keledjian, D. Boneva, B. S. Marasa, and J.-Y. Wang Polyamines are necessary for synthesis and stability of occludin protein in intestinal epithelial cells Am J Physiol Gastrointest Liver Physiol, June 1, 2005; 288(6): G1159 - G1169. [Abstract] [Full Text] [PDF]

				J.-i. Tanaka, M. Matsuzaki, E. Tarusawa, A. Momiyama, E. Molnar, H. Kasai, and R. Shigemoto Number and Density of AMPA Receptors in Single Synapses in Immature Cerebellum J. Neurosci., January 26, 2005; 25(4): 799 - 807. [Abstract] [Full Text] [PDF]

				C. Meier, R. Dermietzel, K. G. V. Davidson, T. Yasumura, and J. E. Rash Connexin32-Containing Gap Junctions in Schwann Cells at the Internodal Zone of Partial Myelin Compaction and in Schmidt-Lanterman Incisures J. Neurosci., March 31, 2004; 24(13): 3186 - 3198. [Abstract] [Full Text] [PDF]

				N. Morel, J.-C. Dedieu, and J.-M. Philippe Specific sorting of the a1 isoform of the V-H+ATPase a subunit to nerve terminals where it associates with both synaptic vesicles and the presynaptic plasma membrane J. Cell Sci., December 1, 2003; 116(23): 4751 - 4762. [Abstract] [Full Text] [PDF]

				C. S. Furman, D. A. Gorelick-Feldman, K. G. V. Davidson, T. Yasumura, J. D. Neely, P. Agre, and J. E. Rash Aquaporin-4 square array assembly: Opposing actions of M1 and M23 isoforms PNAS, November 11, 2003; 100(23): 13609 - 13614. [Abstract] [Full Text] [PDF]

				T. Abe, E. Sugano, Y. Saigo, and M. Tamai Interleukin-1{beta} and Barrier Function of Retinal Pigment Epithelial Cells (ARPE-19): Aberrant Expression of Junctional Complex Molecules Invest. Ophthalmol. Vis. Sci., September 1, 2003; 44(9): 4097 - 4104. [Abstract] [Full Text] [PDF]

				A. Pereda, J. O'Brien, J. I. Nagy, F. Bukauskas, K. G. V. Davidson, N. Kamasawa, T. Yasumura, and J. E. Rash Connexin35 Mediates Electrical Transmission at Mixed Synapses on Mauthner Cells J. Neurosci., August 20, 2003; 23(20): 7489 - 7503. [Abstract] [Full Text] [PDF]

				H. Sasaki, C. Matsui, K. Furuse, Y. Mimori-Kiyosue, M. Furuse, and S. Tsukita Dynamic behavior of paired claudin strands within apposing plasma membranes PNAS, April 1, 2003; 100(7): 3971 - 3976. [Abstract] [Full Text] [PDF]

				J. P. Bilello, E. E. Cable, and H. C. Isom Expression of E-Cadherin and Other Paracellular Junction Genes Is Decreased in Iron-Loaded Hepatocytes Am. J. Pathol., April 1, 2003; 162(4): 1323 - 1338. [Abstract] [Full Text] [PDF]

				K. Tauchi-Sato, S. Ozeki, T. Houjou, R. Taguchi, and T. Fujimoto The Surface of Lipid Droplets Is a Phospholipid Monolayer with a Unique Fatty Acid Composition J. Biol. Chem., November 8, 2002; 277(46): 44507 - 44512. [Abstract] [Full Text] [PDF]

				C. Y. Cheng and D. D. Mruk Cell Junction Dynamics in the Testis: Sertoli-Germ Cell Interactions and Male Contraceptive Development Physiol Rev, October 1, 2002; 82(4): 825 - 874. [Abstract] [Full Text] [PDF]

				Y.-H. Chen, Q. Lu, D. A. Goodenough, and B. Jeansonne Nonreceptor Tyrosine Kinase c-Yes Interacts with Occludin during Tight Junction Formation in Canine Kidney Epithelial Cells Mol. Biol. Cell, April 1, 2002; 13(4): 1227 - 1237. [Abstract] [Full Text] [PDF]

				M. Furuse, M. Hata, K. Furuse, Y. Yoshida, A. Haratake, Y. Sugitani, T. Noda, A. Kubo, and S. Tsukita Claudin-based tight junctions are crucial for the mammalian epidermal barrier: a lesson from claudin-1-deficient mice J. Cell Biol., March 18, 2002; 156(6): 1099 - 1111. [Abstract] [Full Text] [PDF]

				N. P.Y. Chung, D. Mruk, M.-y. Mo, W. M. Lee, and C. Y. Cheng A 22-Amino Acid Synthetic Peptide Corresponding to the Second Extracellular Loop of Rat Occludin Perturbs the Blood-Testis Barrier and Disrupts Spermatogenesis Reversibly In Vivo Biol Reprod, November 1, 2001; 65(5): 1340 - 1351. [Abstract] [Full Text] [PDF]

				J. P. Bilello, W. E. Delaney IV, F. M. Boyce, and H. C. Isom Transient Disruption of Intercellular Junctions Enables Baculovirus Entry into Nondividing Hepatocytes J. Virol., October 15, 2001; 75(20): 9857 - 9871. [Abstract] [Full Text] [PDF]