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Fig. 1. Mouse mammary epithelial cells undergo EMT in response to TGFβ1. (A,B) Phase micrographs of cells from the mouse mammary epithelial cell line NMuMG in the (A) absence or (B) presence of TGFβ1 (5 ng/ml for 1 day). (C,D) Cells were stained for filamentous actin with Texas-red-labeled phalloidin. (C) Untreated cells show a peripheral band of actin filaments, typical of polarized epithelial cells. (D) TGFβ1-treated cells have prominent stress fibers. Reproduced from Maeda et al. (Maeda et al., 2005).