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Figure 4


Fig. 4. (A) Colocalization of internalized FITC-BSA and Alexa-Fluor-568 anti-DC-SIGN F(ab) in a MDDC (scale bar, 10 µm). Representative details of edge (red frame), medial zone (yellow frame), and perinuclear zone regions (blue frame) show non-internalized and internalized DC-SIGN. Three regions in each category (shown by red, yellow and blue boxes) were chosen for quantitative colocalization analysis. (B) Proportion of colocalization of DC-SIGN and internalized FITC-BSA per region (t-test *P<0.001; **P<0.05). (C) Histogram of the ratio of internalized clusters versus surface clusters as a function of distance from the edge of the cell. This analysis is representative of four independent experiments.





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