JCS019513 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. (A-D) Four independent NSOM images corresponding to the composite NSOM image shown in Figure 2 of the main article. The four near-field fluorescence measurements correspond to a Cy5-IL2Rα-labeled FT7.10 cell mapping the full distribution of the receptor on the membrane. The color-coding of the image is the same as in Figs 1 and 2. (E) Central part of the cell with a different imaging contrast, revealing the presence of individual molecules (green and red spots corresponding to single dipole emission). Scale bars, 2 µm.

• Supplemental Figure S2 -

  Fig. S2. Upper panel: Fluorescence intensity histograms of unlabeled cells (background) in blue, cells labeled with Alexa-Fluor-647-conjugated 7A4 24 (IL15Rα; magenta) and isotype-control antibodies (yellow). The Table below shows the corrected mean fluorescence intensity for background, isotype control and IL15Rα.

• Supplemental Figure S3 -

  Fig. S3. (A-C) Confocal microscopy (left panel) and bright-field (right panel) images of K225 cells taken for (A) unlabeled cells, i.e. detection of autofluorescence background; (B) an isotype control labeled with Alexa-Fluor-647; and (C) Il15Rα (Alexa-Fluor-647−7A4 24).