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Figure 1


Fig. 1. Dynactin complex is enriched at the P2-EMS border prior to and during EMS and P2 spindle rotation. Multi-photon time series of embryos expressing (A) DNC-1::GFP (supplementary material Movie 1); (B) DNC-2::GFP; dnc-2 3'UTR RNAi; and (C) ARP-1::GFP; arp-1 3'UTR RNAi show the accumulation of dynactin along the P2-EMS border (marked by white arrows). 3' UTR RNAi was used to improve the image contrast of DNC-2::GFP and ARP-1::GFP (see text). Notice that dynactin also localizes to centrosomes, serving as a marker for the spindle positioning. (A') Enlarged region between the EMS centrosome and the P2-EMS accumulation in a DNC-1::GFP embryo. Arrowhead points to a dynactin-labeled astral MT plus-end that terminates at the accumulation site. Scale bar: 10 µm.





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