QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

Help viewing high resolution images Return to article

Fig. 6. Cdc24 overexpression bypassed the requirement of Cdk5 for polar growth. (A) Overexpression of cdc24 induces polar growth in cdk5^ts cells. Wild-type and cdk5^ts cells, as well as derivates carrying constructions for overexpression under the crg1 promoter (repressed in glucose, induced in arabinose) of rac1, rac1^Q61L and cdc24 were grown at the permissive temperature until OD₆₀₀=0.2 and then shifted to the restrictive temperature (34°C) in arabinose-containing complete medium for 5-6 hours. (B) Time series of cell poles of a cdk5^ts myo5-GFP cell as well as a cdk5^ts myo5-GFP cell overexpressing cdc24 at the restrictive temperature. Note that in the cdk5^ts cell the GFP-Myo5 accumulation at the growth region undergoes rapid rearrangement at the restrictive temperature, but when overexpressing cdc24, the GFP-Myo5 signal remains stationary. Elapsed time is given in minutes:seconds. Bars, 3 µm. (C) Lack of suppression of temperature-sensitive growth of cdk5^ts cells after cdc24 overexpression. Wild-type and cdk5^ts cells carrying a construction overexpressing cdc24 under the control of the crg1 promoter were spotted in inducing (YPA) and non-inducing (YPD) conditions and incubated at permissive (22°C) and restrictive (34°C) conditions.

Return to article