spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 5


Fig. 5. Role of TNF{alpha} and ROS in hematopoietic suppression. (A) The ROS scavenger NAC or deletion of Tnfr1 gene rescued progenitor growth. WT, Fancc–/– mice or their littermates lacking Tnfr1 were injected with PBS or TNF{alpha} (0.1 mg/kg per day) for 2 consecutive days. Mice were injected with NAC (1 mg/mouse per day) 30 minutes before and after TNF{alpha} injection. Twenty-four hours later, BM cells were isolated and subjected to clonogenic assay. Data shown represent the mean ± s.d. of the total number of colonies from three independent experiments; *P<0.05. (B) Anti-oxidant NAC or deletion of the Tnfr1 gene restored HSC self-renewal ability. 2x106 BM mononuclear cells isolated from the mice described in A were transplanted together with 1x106 competitor cells from B6.BoyJ mice (CD45.1+) into lethally irradiated recipient mice; long-term engraftment was evaluated 16 weeks after transplantation. Data represent the mean ± s.d. of three independent experiments with three recipients per group for each experiment. *P<0.05.





Right arrow Return to article