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Figure 2


Fig. 2. Analysis of differentiation, apoptosis and cell cycle of HSCs and progenitor cells from TNF{alpha}-treated mice. (A) TNF{alpha} did not affect the differentiation of long-term repopulating HSCs. Donor-derived (CD45.2+) leukocytes in peripheral blood of recipient mice transplanted with the indicated BM cells were stained with antibodies that recognize Gr-1 and Mac-1 or B220 and CD3 or Ter119. Data are expressed as mean ± s.d. of three independent experiments, each with three recipients (nine mice per group). (B) TNF{alpha} induced apoptosis in BM LSK cells of mice. BM cells from PBS- or TNF{alpha}-treated WT and Fancc–/– mice were stained with lineage maker antibodies together with Sca-1 and c-Kit antibodies, and then with annexin V. Percentages of apoptosis in the LSK population were analyzed by flow cytometry. Numbers in the quadrants indicate percent of cells labeled for 7-AADlowannexin V+ or 7-AADhighannexin V+. (C) BM LSK cells from TNF{alpha}-injected Fancc–/– mice show increased G2-M arrest. BM cells from PBS- or TNF{alpha}-treated WT and Fancc–/– mice were gated for LSK cells and stained with propidium iodide (PI) followed by analysis for cell cycle distribution. Shown are representative flow cytometric presentations of three independent experiments. Numbers in plots indicate percent of cells in G2-M phases.





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