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Figure 2


Fig. 2. RhoB T19N and Q63L mutants impair CXCR2-mediated chemotaxis. (A-C) Boyden chamber assay assessing chemotaxis of HEK293 cells stably expressing CXCR2 and transiently transfected with empty vector, Myc-RhoB T19N or Myc-RhoB Q63L. A twofold reduction in CXCR2-mediated chemotaxis was observed when Myc-RhoB T19N and Myc-RhoB Q63L mutants were expressed. No effect on chemotaxis was observed when Myc-RhoB WT was expressed. CXCR2-mediated chemotaxis of cells expressing Myc-RhoB T19N (A), Myc-RhoB Q63L (B) and Myc-RhoB WT (C). The graphs display numbers of cells from twenty separate fields ± s.e.m., using the 20x objective lens. + and * indicate significant differences (P<0.01 and P<0.005; Student's t-test) of vector-transfected cells versus cells transfected with Myc-RhoB T19N or Myc-RhoB Q63L. Data shown are representative from three separate experiments.





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