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Figure 1


Fig. 1. RhoB is activated upon CXCL8 stimulation. Western blot analysis with anti-RhoB antibody of input lysates (Total RhoB) and GST-TRBD bound RhoB (GTP-RhoB) separated by SDS-PAGE. HEK293 cells stably expressing CXCR2 were transiently transfected with Myc-WT RhoB or Myc-Q63L RhoB mutant. Cells transfected with Myc-Q63L RhoB were stimulated with vehicle. Cells transfected with Myc-WT RhoB were stimulated with 100ng/ml EGF for 60 minutes (EGF), vehicle (Unt), or 100 ng/ml CXCL8 for 5 minutes, 30 minutes or 60 minutes. Lysates were incubated with GST-TRBD (rhotekin Rho-binding domain) to isolate GTP-bound RhoB. Data shown are representative from three separate experiments. Induction at 5-minute and 60-minute time points when normalized to total RhoB was 1.6-fold and 1.1-fold, respectively, in the experiment shown here.





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