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Figure 9


Fig. 9. LMB does not affect the ability of speckles to accumulate snRNPs. HeLa cells expressing PA-GFP-SmB were treated with LMB for 4 hours. A region of the nucleus containing a CB (circle in A, arrow in B) was repeatedly activated with a 406 nm laser and short time-lapse sequences were taken between each bleach event to monitor the spread of PA-GFP-SmB in the nucleus. The signal rapidly accumulated in speckles throughout the nucleus (arrowheads in C). Image A shows the cell before any laser activation, image B is immediately after the first activation event and image C is immediately after the tenth activation event. Bars, 11 µm.





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