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Figure 1


Fig. 1. Incorporation of FP-tagged Sm proteins into snRNPs for FRET analyses. (A) Assembly of Sm subcomplexes D1/D2 and E/F/G onto snRNA forms a stable subcomplex. Subsequent binding of the D3/B complex completes the Sm core domain and places SmD1 and SmB next to each other (adapted from Kambach et al., 1999b, with permission from Elsevier). (B) Transfection of cells with different combinations of FP-tagged Sm proteins is predicted to produce complexes containing YFP-SmB next to CFP-SmD1, resulting in FRET interaction, or YFP-SmB and CFP-SmB in separate complexes, producing no FRET and acting as a negative control.





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