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Figure 6


Fig. 6. Dumbbell-like structures in G2-arrested cells treated with a topoisomerase II inhibitor. (A) Cells harboring the recombinant EBV were synchronized at the G1-S boundary and then released. Cell cycle progression, in the presence or absence of ICRF-193, was monitored by FACS analyses. (B) A representative image (out of more than 20 acquired images) of ICRF-193-treated cells (10 hours after release) that were processed for simultaneous FISH and immunofluorescence. Note many dumbbell-like structures consisting of EBNA1 doublets (red) and EBV genome signals (green). Merged images are also shown. Bar, 5 µm.





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