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Figure 5


Fig. 5. Simultaneous visualization of EBNA1 and EBV episomes in G2-phase-enriched cells. (A) Simultaneous FISH and immunofluorescence images of two interphase cells (out of more than 50 acquired images of interphase nuclei harboring EBNA1 doublets). Cells were harvested at 6 hours after release and processed for simultaneous FISH and immunofluorescence. The signals of EBV episomes (green signals revealed by FISH) and those of EBNA1 protein (red signals revealed by immunofluorescence) are shown as merged images. Chromosomes are counterstained with DAPI (left panels). Higher magnification images of the EBNA1 double-dots, merged with the signals of EBV episomes, are shown in the sub-panels (1-6). Scale bars are as indicated. (B) Simultaneous FISH and immunofluorescence images of two representative G2-PCCs (out of more than 20 acquired images of G2-PCCs). Higher magnification images of the EBNA1 double-dots, merged with the signals of EBV episomes, are also shown in the accompanying sub-panels. Scale bars are as indicated.





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