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Figure 5


Fig. 5. Effect of rMIS and YWK-II antibody on mouse sperm viability and ERK activation. (A) Sperm viability in response to treatment with rMIS and YWK-II antibody. Sperm were preincubated with control IgG or YWK-II antibody (Ab) for 15 minutes prior to addition of equal volume of PBS or rMIS (0.075 µM) followed by further incubation for 10 minutes or 30 minutes. Sperm were mixed with 0.04% Trypan Blue for evaluation of viability. The data are the mean percentage of viable sperm from three experiments (*P<0.05). (B) Effect of YWK-II antibody on rMIS-induced ERK1/2 activation in mouse sperm. Sperm were preincubated with control IgG or YWK-II antibody for 15 minutes prior to addition of rMIS (0.075 µM) followed by further incubation for 30 minutes. The proteins were extracted for western blot analysis with total ERK1/2 as a loading control.





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