spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 4


Fig. 4. Effect of rMIS on cell viability and apoptotic activity in EGPF-YWK-II-overexpressing CHO cells. (A) Effect of rMIS and YWK-II proteins on cell viability. Cells transfected with EPGFP-YWK-II protein or EGFP and non-treated CHO cells were treated with different concentrations of rMIS for 3 days and assayed using the MTT method. The values obtained with the control untreated groups were set at 100% (*P<0.01). (B) Effect of rMIS on p53 levels in CHO cells. The established EGFP-YWK-II-overexpressing (YWK-II) and EGFP-transfected (EGFP) CHO cells and non-transfected CHO controls were cultured to confluence and incubated in serum-free medium for 24 hours prior to stimulation with 0.35 nM rMIS for 5 minutes. (C) Effect of rMIS on caspase-3 levels in YWK-II-overexpressing CHO cells. The cells were treated with 0.35 nM rMIS in the presence of YWK-II antibody (YWK-II Ab, 150 ng) or control IgG (150 ng) followed by western blot analysis of pro-caspase-3, with beta-actin as the loading control.





Right arrow Return to article