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Figure 2


Fig. 2. Confirmation of YWK-II protein expression in the CHO cell line by western blotting. Electrophoresis pattern of lysates of CHO cell, CHO cells stably transfected with pEGFP-N1-YWK-II (pEGFP-YWK-II) or pEGFP-N1 (pEGFP), stained with rabbit YWK-II antibody. The total protein loaded on the gel was 20 µg. Only the 67 kDa protein in lysate obtained from stable CHO cells transfected with pEGFP-N1-YWK-II was detected by YWK-II antibody (molecular mass of recombinant EGFP-YWK-II protein, ~67 kDa).





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