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Figure 1


Fig. 1. Localization of the EGFP-YWK-II protein on the cell membrane visualized by laser confocal scanning microscopy. (a-c) CHO cells transiently transfected with pEGFP-N1 were fixed, permeabilized and stained with propidium iodide (PI). (a) EGFP-overexpressing CHO cells (green). (b) CHO cells stained with PI to delineate the nucleus (red). (c) superimposed image of a and b. (d-f) CHO cells transiently transfected with pEGFP-N1-YWK-II were fixed, permeabilized and stained with PI. (d) EGFP-YWK-II-overexpressing CHO cells (green). (e) CHO cells stained with PI to delineate the nucleus (red). (f) Superimposed image of d and e. (g-i) CHO cells were transiently transfected with pEGFP-N1-YWK-II, fixed and not permeabilized. (g) EGFP-YWK-II-overexpressing CHO cells (green). (h) EGFP-YWK-II protein overexpressed on the cell membrane with rabbit YWK-II antiserum as the primary antibody and TRITC-conjugated goat anti-mouse IgG as the second antibody (red). (i) Superimposed image of g and h. (j-l) CHO cells were transiently transfected with pEGFP-N1-YWK-II, fixed and not permeabilized. (j) EGFP-YWK-II-overexpressing CHO cells (green). (k) EGFP-YWK-II-overexpressing cells using rabbit preimmune serum as the primary antibody and TRITC-conjugated goat anti-mouse IgG as the secondary antibody showing negative staining. (l) Superimposed image of j and k.





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