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Figure 4


Fig. 4. TbLC1 is required for stable outer arm dynein assembly. (A) Electron micrographs of uninduced (–Tet) and induced (+Tet, 30 hpi) whole cell TbLC1 mutant flagellar cross-sections. Arrows indicate examples of positions on doublets where outer dynein arms are present (– Tet) or absent (+ Tet). The table shows the breakdown of how many arms were missing from uninduced and induced cells at the indicated time points. Increasing the time of induction does not affect the extent of outer arm loss. (B) Analysis of central pair orientation in TbLC1 knockdown cells. Central pair orientation was determined as described previously (Ralston et al., 2006). n=22-37 sections (–Tet) or 30-40 sections (+Tet). The percentage of sections having central pair orientation outside the range observed in associated –Tet samples are indicated.





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