JCS004846 Supplementary Material

Files in this Data Supplement:

• Movie 1 -

  Movie 1. Loss of TbLC1 causes flagellar beat reversal and backward locomotion. Ten-second real-time movie captures of TbLC1 and trypanin mutant cells. Backward motility that results from reverse flagellar beat in TbLC1 mutants. Note that the cell is moving with the flagellum tip trailing.

• Movie 2 -

  Movie 2. The trypanin mutant cell used for still images in Fig. 3C and demonstrates that these mutants retain tip-to-base flagellar beat despite their tumbling phenotype.

• Movie 3 -

  Movie 3. The TbLC1 mutant cell used for the still images in Fig. 3C, showing propagation of flagellar beat base-to-tip. Cells stuck to glass slides were used to illustrate flagellar beat propagation independently of cell movement.