|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
|
Fig. 5. Rescue of FHL3 siRNA knockdown phenotype. (A) siRNA-transfected C2C12 Vector (2) or HA-FHL3 (2) cell lines were harvested 48 hours post transfection (0 hours differentiation) and lysates immunoblotted for FHL3, HA or actin. Western blots are representative of four experiments. (B) siRNA-transfected C2C12 Vector (2) or HA-FHL3 (2) cell lines were differentiated for 72 hours and stained with anti-MHC antibodies (green) and propidium iodide (red), and imaged by confocal microscopy. Images are representative of four independent experiments. Bar, 20 µm. (C) siRNA-transfected C2C12 Vector (2) and HA-FHL3 (2) cell lines were harvested following 24 hours of differentiation and lysates immunoblotted for myogenin or actin. Western blots are representative of three experiments. (D) The mean relative level of myogenin protein during siRNA-mediated knockdown of endogenous FHL3 in Vector (2) or HA-FHL3 (2) cell lines was quantified by densitometry expressed relative to actin loading and standardised to vector levels set at 1 arbitrarily. C2C12 cells transfected with Vector (2) are represented by white bars, HA-FHL3 (2) by black bars, error bars represent ± s.e.m. (n=3, *P<0.05, **P<0.01). siRNA transfected is shown below figure as indicated.
|
