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Figure 8


Fig. 8. NO145 protein in bovine oocytes. (A) Immunoblot on proteins extracted from isolated bovine oocytes, probed with hsNO145-1A antibody. (B-C'') Immunolocalization in the nucleus of bovine oocytes using hsNO145-specific antibodies (green) and DNA counterstaining (red). (B) Overview of an oocyte surrounded by the cumulus cell layers. (B') Magnification of this nucleus shows that NO145 protein is enriched in dot-like structures (arrowheads) associated with dense chromatin but not with the partly `vacuolated' nucleolus (arrow). (C-C'') Magnification of another GV showing NO145 protein in granular structures (arrowheads) that are not closely associated to nucleoli (arrows). Bars, 20 µm (B) and 10 µm (B' and C).





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