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Fig. 7. The human orthologue of the NO145 protein. (A) Sequence comparison between human NO145 (hsNO145) and its Xenopus homolog (X.l. NO145). Identical amino acids (aa) are marked by vertical bars and are indicated in bold letters. Peptide sequences used for generating antibodies are indicated by boxes, the putative nuclear localization signal is shaded grey and putative sequence motifs involved in protein degradation are indicated in red. (B) Autoradiogram of SDS-PAGE-separated rabbit reticulocyte lysate after in vitro transcription and translation in the presence of the pBT-hsNO145. Bars indicate the position of reference proteins: 205, 116, 97.4 and 66 kDa (from top to bottom). (C,C') Tissue protein lysates of human origin (C; ovary, testis, intestine; shown in lanes 1-3, respectively, after Coomassie Blue staining) were analyzed by western blotting (C') using NO145-specific antibodies. A parallel blot was probed for vimentin using the mAb Vim3B4 (Herrmann et al., 1989). Reference proteins are the same as in B. (D-D'') Immunofluorescent localization of NO145 protein on paraffin sections through human ovary using hsNO145-specific antibodies. (D) Phase-contrast micrograph; (D') DAPI staining; (D'') corresponding immunofluorescence micrograph using hsNO145-5 antibody. Bar, 50 µm.

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