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Figure 5


Fig. 5. NO145 protein and its mRNA during X. laevis embryogenesis. (A) Immunoblot of SDS-PAGE-separated total proteins isolated from stage VI oocytes and from embryos of stages 1-25, probed with NO145-specific antibodies (top panel) and antibodies against the nucleolar protein NO66 (bottom). (B) Total RNA from stage VI oocytes and embryos of stages 1-10 were separated by agarose gel electrophoresis. The corresponding northern blot was hybridized with a random prime-labeled NO145-specific probe. (C) RACE-PAT assays performed on total RNA from X. laevis oocytes (VI), embryos (stages 1-25), and eggs (E) with NO145-specific primers. The resulting PCR products were separated on a 2% agarose gel and stained with ethidium bromide. Re-amplified PCR products from embryonic stages 12, 15 and 25, respectively, are shown in the box below. Bars over the lane number indicate midblastula transition (MBT; fraction 8 in A,B,C).





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