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Figure 1


Fig. 1. Karyoskeletal protein NO145 in Xenopus oocytes and eggs. (A) Laser scanning confocal microscopy showing double label immunolocalization on spread preparations of GV contents of X. laevis stage VI oocytes. The specific localization of NO145 protein in the nucleolar cortex (green) and of nucleolar protein NO38/B23 (red) in the granular component are shown in the merged picture. Bar, 15 µm. (B,C) Synthesis of NO145 protein and its mRNA in X. laevis oocytes and eggs. (B) Western blot of total proteins of one oocyte (VI) and egg (E), probed with antibody NO145-H or antibody X8 directed against protein NO38/B23. (C) Northern blot of total RNA from stage VI oocytes (VI) and eggs (E) was hybridized with a random prime-labeled NO145-specific probe (left panel); the ethidium bromide-stained gel serves as a loading control (right panel). RNA size markers of 4, 3, 2, 1.5 and 1 kb are indicated.





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