spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 6


Fig. 6. (A) Immunofluorescence images of actin (a,c,e,g,i,k) and vinculin (b,d,f,h,j,l) in REFs adherent to 10 µg/ml fibronectin (a,c) or to a fragment containing the central cell binding region of fibronectin (110-FN) (c-l) for 2 hours. The heparan sulphate binding fibrillin-1 fragments (10 µg/ml) PF9 (e,f), an RGD-to-RDG mutant PF9 (g,h), PF10 (i,j), or the heparan sulphate binding region of fibronectin (HepII) (k,l) were added in solution for 30 minutes before fixing. (B) The table shows quantification of the number of cells on FN, 110, or 110+fibrillin-1 fragments that express vinculin-containing and non-vinculin-containing focal plaques (±s.d.). (C) Immunofluorescence of actin (a) and vinculin (b) in REFs adherent to 10 µg/ml of 110-fibronectin for 2 hours. Soluble 10 µg/ml heparan sulphate binding mutant R1691A/R1692APF9 was added for 30 minutes before fixing.





Right arrow Return to article