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Figure 5


Fig. 5. (A) Immunofluorescence images of actin (a,c,e,g) and vinculin (b,d,f,h) in MEFs adherent to 10 µg/ml PF8 (a-h) for 2 hours. 10 µg/ml of the heparan sulphate binding fragments of fibrillin-1 PF9 (c,d), PF10 (e,f) and PF11 (g,h) were added in solution for 30 minutes before fixing. Quantification revealed that 9% of cells on PF8, 57% of cells on PF8 + soluble PF10, 38% of cells on PF8 + soluble PF9 and 11% of cells on PF8 + soluble mutant PF9 were positive for vinculin and focal adhesions. (B) Immunofluorescence images of actin (a) and vinculin (b) in MEFs adherent to PF8 in the presence of soluble 10 µg/ml heparan sulphate binding mutant R1691A/R1692A PF9.





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