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Figure 2


Fig. 2. (A) Dose-dependent HDF attachment to fibrillin-1 fragments PF8 (squares), PF11 (diamonds) and the central cell binding region of fibronectin (50K-FN) (triangles). (B) HDF attachment to 10 µg/ml PF11, PF8 or BSA-blocked plastic in the presence (shaded) or absence (open) of the beta1-integrin-activating antibody TS2/16. Data were statistically analysed using unpaired Student's t-tests (GraphPad Prism 2.0). Error bars represent the s.d. of the three experiments. (C) Fluorescence images of actin filaments in phalloidin-stained spread fibroblasts on fibronectin, PF11, PF8 and PF9. There were no spread cells in the BSA control (not shown). (D) Cumulative distance travelled by cells adherent to 50K-FN (solid line), PF8 (long dashed line) or PF11 (short dashed line), as measured by time lapse microscopy. Bar, 50 µm.





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