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Figure 1


Fig. 1. (A) Domain organisation of recombinant fibrillin-1 fragments, indicating the levels of cell attachment each fragment supports (+, low attachment; +++++, high attachment) and heparin-binding sites. (B) SDS-PAGE analysis (4-12% Bis-Tris gels) of fibrillin-1 fragments WT PF14, RGAPF14, 19-20PF14, 19-20-21-20PF14, 3-4-5-6PF14, 19-4-5-6PF14 and 3-20-21-6PF14, under non-reducing (i) and reducing (ii) conditions and after PNGaseF digestion (iii). (C) SDS-PAGE analysis (4-12% Bis-Tris gels) of fibrillin-1 fragments WT PF9, R1691APF9, R1692APF9, R1697APF9, R1752APF9, R1691A/R1692APF9 and R1697A/R1752APF9 under non-reducing (i) and reducing (ii) conditions and after PNGaseF digestion (iii).





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