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Figure 5


Fig. 5. Twist-1 protein stability is increased by the formation of a heterodimer complex with E47. (A) Myc-tagged Twist-1 and E47 constructs and GFP expression vector were transfected into COS-7 cells. Western blot analysis was performed with anti-Myc antibody. To show transfection efficiency, GFP protein was also detected by western blot. (B) COS-7 cells were transfected with the Flag-tagged Twist-1, Myc-tagged E47 and/or Myc-tagged Id1. At 24 hours after transfection, cells were pulsed with [35S]methionine and cysteine for 3 hours, and chased with unlabeled medium for the indicated times. Labeled cell lysates were immunoprecipitated using anti-Flag antibody. Flag-tagged Twist-1 was visualized using SDS-PAGE.





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