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Figure 4


Fig. 4. Twist-1 can interact with HDAC1 and Smad4. (A) MC3T3-E1-WT and MC3T3-E1-Tw1 cells were treated with BMP2 (300 ng/ml) for 24 hours. Lysates were immunoprecipitated using anti-Flag antibody and blotted with anti-Smad4 and HDAC1 antibody. 10% In, 10% of input; IP, immunoprecipitated fraction. (B) MC3T3-E1-Tw1 cells were treated with BMP alone [TSA (–)] or the mixture of BMP and TSA (82.5 µM) [TSA (+)]. After 24 hours, total RNA was extracted and the expression of ALP, Runx2 and osteopontin was quantified by real-time PCR. The expression levels were normalized by GAPDH, and the ratio was shown in each sample. Data are presented as mean ± s.d. of triplicate samples (*P<0.05).





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