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Figure 7


Fig. 7. Oocytes from T-synF/FMgat1F/F:ZP3Cre double mutant (DM) females lack core-1-derived O-glycans and complex and hybrid N-glycans. (A) The ZP of oocytes in T-synF/+Mgat1F/+:ZP3Cre control ovaries bound both L-PHA-FITC and PNA-FITC whereas the ZP on oocytes of DM ovaries (arrows) did not bind either lectin. Images are overexposed to reveal unstained oocytes present in DM ovaries and follicles are size-matched for developmental stage. (B) Monoclonal antibodies to ZP1, ZP2 and ZP3 bound to control and DM ZP. The ovaries were from three previously mated control and 3 DM females and represent six control and ten DM females. Asterisks indicate females that gave birth. Formalin fixation was used for lectin staining whereas both formalin (f) and Bouins (b) fixed ovaries were used for ZP staining.





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