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Figure 6


Fig. 6. Eggs from T-synF/FMgat1F/F:ZP3Cre DM females lack core-1-derived O-glycans and complex and hybrid N-glycans. (A,B) Phase-contrast micrographs showing persistent cumulus cells attached to T-syn–/–Mgat1–/– eggs after hyaluronidase treatment. (C,D) Higher magnification of T-syn–/–Mgat1–/– eggs with less cumulus attached revealed a thin fragile zona. (E,F) PNA-FITC did not bind to T-syn–/–Mgat1–/– eggs. Similar data were obtained in three experiments with eggs from 12 T-synF/FMgat1F/F:ZP3Cre females. (G,H) L-PHA-FITC did not bind to T-syn–/–Mgat1–/– eggs. Similar data were obtained in four experiments from 16 T-synF/FMgat1F/F:ZP3Cre females. (I,J) Con A-Rho staining was enhanced in T-syn–/–Mgat1–/– eggs consistent with increased oligomannosyl N-glycans.





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