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Figure 5


Fig. 5. Eggs from T-synF/F:ZP3Cre females lack core-1-derived O-glycans. (A,B) PNA-FITC bound to wild-type but not T-syn–/– eggs. Similar data were obtained in five experiments with eggs from 12 T-synF/F:ZP3Cre females. (C,D) Anti-Tn antibodies bound to the Tn antigen on T-syn–/– eggs, but poorly to wild-type eggs. Similar results were obtained in four experiments from 16 T-synF/F:ZP3Cre females. (E,F) L-PHA-FITC bound to mutant and wild-type eggs equivalently. Similar results were obtained in three experiments from seven T-synF/F:ZP3Cre females. (G,H) Western analyses of ZP1 and ZP3 from control and T-synF/F:ZP3Cre ovaries before and after digestion with N-glycanase.





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