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Figure 2


Fig. 2. Generation of the T-syn allele and oocyte-specific deletion. (A) Diagram of T-syn and Mgat1 floxed and deleted alleles, and the ZP3Cre transgene with positions of primers used in genotyping. The T-syn-, Mgat1- and Cre-coding regions are shaded, and the promoter region for ZP3 is an open box. (B) Mouse genotype was determined using PCR of genomic tail DNA. Lane M, 1 kb plus markers. (C) O-glycan synthesis. T-synthase transfers Gal to GalNAc on Ser/Thr generating core 1. Subsequently GlcNAc may be added to the GalNAc to generate core 2. Core 3 and 4 structures have not been detected on mouse ZP3 (Boja et al., 2003; Chalabi et al., 2006). (D) Relationship of genotype of mutant females to genotype of eggs. (E) Diagram of complex N-glycan structure showing that the deletion of the Mgat1 gene generates oligomannosyl N-glycans. Open square, GalNAc; open circle, Gal; black square, GlcNAc; black diamond, sialic acid; triangle, fucose; black circle, mannose.





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