spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 3


Fig. 3. Normal disassembly of centrosome-induced asters in the absence of functional p97. (A) CSF-extract was pre-treated with murine wild-type p97, the E305Q-E578Q mutant p97-QQ (both at 1 mg/ml final concentration) or reference buffer. Thirty minutes after addition of human centrosomes, meiotic exit was triggered and Rhodamine-labelled microtubules were visualized. Micrographs show representative centrosomes 0 and 60 minutes after release into interphase. Relative to the top row of images, the bottom row of images are overexposed. Bars, 10 µm. (B) CSF-extracts were depleted of Ufd1 and Npl4 or mock-depleted, and combined with centrosomes to induce aster formation (released and analyzed as in A). (C) Analysis of depleted extracts from B, carried out as described in Fig. 1A.





Right arrow Return to article