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Figure 2


Fig. 2. Spindle disassembly is not delayed in the absence of functional p97. (A) CSF-extracts pre-incubated with murine wild-type p97, the E305Q-E578Q mutant p97-QQ (both at 1 mg/ml final concentration) or reference buffer for 30 minutes at 4°C were supplemented with Rhodamine-tubulin and 500 sperm/µl. Following release (t=0 minutes) kinetics of spindle disassembly were monitored. (B) Ufd1-Npl4-depleted CSF extract from 1A was supplemented with soluble anti-Npl4 antibody. Kinetics of spindle disassembly relative to mock-depleted extract plus unspecific IgG or to untreated extract plus reference buffer were monitored as in A.





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