spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 5


Fig. 5. Effect of rotenone treatment on mitochondrial {Psi}{Delta}, cytosolic ROS and matrix redox potential. (A) Determination of {Psi}{Delta} in MRC5 cells treated with increasing concentrations of rotenone, measured with the JC1 probe and expressed as the ratio of red to green fluorescence. (B) Determination of the cytosolic ROS concentration by fluorescence intensity of the H2-DCFDA probe, expressed as the percentage of control value. Results are given as the mean ± s.d., n=3. (C) Measurement of the redox potential of the mitochondrial matrix in MRC5 cells treated with increasing rotenone concentrations, measured with redox-GFP and expressed as the percentage of the fluorescence intensity (emission ratio of 511 nm, excitation set at 400 nm and 480 nm) measured in control cells (not shown). *P<0.05, statistically significant change compared with the control.





Right arrow Return to article