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Fig. 1. Silencing of DRP1 in HeLa cells results in alterations of the mt-network morphology. (A-C) mt-network organization was observed in (A) wild-type HeLa cells, and (B) non-induced and (C) induced HeLa cells carrying the tet-inducible siRNA vector. Notice the budding of mt-network around the nucleus and the long, poorly ramified, tubules in C. Bars, 10 µm. (D) Western blots performed with 10 µg of cell-lysate proteins obtained from these cells.