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Figure 6


Fig. 6. Colocalization analyses of Hh components when endocytosis is blocked. Salivary gland cells expressing the dominant negative form of Shi, ShiK44A, or Rab5, Rab5SN stained for Ci and Ptc or Ci and Smo (and Sxl in females). Female glands expressing ShiK44A stained for Sxl, Smo and Ci (A-C) or Sxl, Ptc and Ci (G-I). Male glands expressing ShiK44A stained for Smo and Ci (D,E) or Ptc and Ci (J,K). `Col.' panels (A'-C',F,G'-I',L) show only pixels common to the indicated proteins. (M-O,S-U) Female glands expressing Rab5SN stained for Sxl, Smo and Ci (M-O) or Sxl, Ptc and Ci (S-U). `Col.' panels (M'-O',P'-R') show only pixels common to both proteins. All sections are from near the plasma membrane. Percentages of all colocalizations are given in Fig. 7.





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