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Figure 4


Fig. 4. ATP-{gamma}-S-induced AKT and ERK phosphorylation is Ca2+ and EGFR dependent. Growth-factor-starved THCE cells were pretreated with either 50 µM Ca2+ chelator BAPTA-AM (A) or 1 µM EGFR inhibitor AG 1478 (B) for 1 hour and then stimulated with 100 µM ATP-{gamma}-S for 30 minutes. Cell lysates were subjected to western blotting with antibodies against phosphorylated AKT (P-AKT), phosphorylated ERK1/2 (P-ERK), AKT and ERK2.





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