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Fig. 7. Loss of EHD1 expression results in impaired cell motility. Equal numbers of Ehd1+/+ MEF (A,C,E) and Ehd1–/– MEF cells (B,D,F), were plated on fibronectin (FN)-coated coverslips (C,D) or vitronectin (VN)-coated coverslips (E,F). Scratches of 
700 µm were introduced on the coverslips, and the cells were either fixed immediately (A,B) or after an additional 12-hour incubation period (C-F). Cells were visualized with fluorochrome-labeled wheat germ agglutinin and confocal microscopy. The micrographs depicted are representative ones from six independent experiments. Ehd1+/+ and Ehd1–/– MEF cells were plated on fibronectin-coated coverslides at sub-confluent concentrations (G,H) and scratched as in A-F. Eight hours after the scratch, cells were then pulsed with 9EG7 anti-
1 integrin antibody for an additional hour to visualize 1 integrins in the migrating cells. Arrows indicate the direction of the migration, and the scratch region is indicated by broken lines. This experiment is a representative one of five independent experiments. Bars, 200 µm (A-F); 10 µm (G,H).
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