QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

Help viewing high resolution images Return to article

Fig. 6. Slower disassembly of focal adhesions in Ehd1^–/– MEF cells. MEF cells were plated on fibronectin-coated coverslips, serum-starved and fixed (A,B; I,J) or treated with 10 µM nocodazole (NOC) for 15 minutes at 37°C (C-H; K-P). For some coverslips, NOC was washed out by incubating the cells in starvation media in the absence of the drug for an additional 15 minutes (E,F; M,N) or 30 minutes (G,H; O,P). After fixation, cells were immunostained with either mouse anti-tubulin (A-H) or mouse anti-paxillin (I-P). (Q) Quantification of focal-adhesion length in Ehd1^–/– and Ehd^+/+ cells was done by using Zeiss LSM 5 Pascale software. The number of focal adhesions measured is indicated, and Student's t-tests were applied to determine the significance of differences in mean focal adhesion lengths. Differences exhibiting a high degree of significance (P<0.0001) are noted with asterisks. This is a representative experiment from four independent experiments. Bars, 10 µm.

Return to article