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Figure 4


Fig. 4. Increased levels of activated beta1 integrins but lower overall levels of beta1 integrins are expressed on the plasma membrane of Ehd1–/– MEF cells. MEF cells grown on uncoated (A-C) or fibronectin-coated (A; FN) plates were harvested by brief trypsinization (A; Trypsin and B,C) or scraping (A; Scrape), fixed and subjected to flow cytometry analysis under non-permeabilizing conditions with secondary antibody only (A-C) or first with 9EG7 antibodies that preferentially recognize ligand-bound beta1 integrins (BD Pharmingen) (A), conformation-independent beta1 integrin antibodies (MB1.2, Chemicon) (B), anti-LAMP1 antibodies (C) or antibodies directed against the epidermal growth factor (EGF) receptor (C) prior to secondary antibodies. The graphs depicted are representative of 4-6 independent experiments. +/+, Ehd1+/+ MEF cells; –/–, Ehd1–/– MEF cells.





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