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Figure 7


Fig. 7. The constitutively active mutant of ARF6, ARF6Q67L, prevents inhibition of cortical actin formation by centaurin-{alpha}2 in EGF-stimulated cells. HeLa cells were transiently transfected with either GFP or GFP-centaurin-{alpha}2 with ARF6Q67L-HA or ARF1Q71L-HA. After 2 days, the cells were serum starved before stimulation with EGF. The cells were then fixed, immunostained with an anti-HA antibody and Rhodamine-conjugated phalloidin (for actin), and imaged using a confocal microscope. The images are representative of 90-95 transfected cells from three experiments.





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