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Figure 6


Fig. 6. Effect of centaurin-{alpha}2 and its mutants on ARF6 localisation and cortical actin formation in EGF-stimulated cells. HeLa cells were transiently transfected with GFP, GFP-centaurin-{alpha}2 or its mutants (R53C; DM), with or without ARF6-HA. After 2 days, the cells were serum starved and stimulated with EGF (0.1 µg/ml). The cells were then fixed, immunostained with an anti-HA antibody or Rhodamine-conjugated phalloidin (for actin) and imaged using a confocal microscope. The images are representative of 175-180 transfected cells from three experiments.





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