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Fig. 6. Characterization of the signaling events in Inv-Dsg2 epidermis. Total protein lysates of wild-type and Inv-Dsg2 transgenic adult skin were analyzed by western analysis to assess activation (phosphorylation) states of signaling intermediates. Immunoblotting for actin, pan cytokeratin (CK) and
-tubulin expression showed equal loading of lanes.