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Figure 4


Fig. 4. Changes in expression patterns of cornified envelope and keratin proteins in Inv-Dsg2 mice. (A) Immunofluorescent staining of 3-month old adult wild-type and Inv-Dsg2 transgenic mouse skin for cytokeratin (CK14, CK10 and CK6), involucrin (Inv), filaggrin (Fil), and loricrin (Lor). CK14 was observed only in the basal layer of the control but extended into the suprabasal epidermal layers of Inv-Dsg2 skin. Increased expression of CK10 was observed in the Inv-Dsg2 epidermis. CK6 expression was observed in the interfollicular epidermis in the Inv-Dsg2 mice. Suprabasal Dsg2 expression was associated with expression of involucrin, filaggrin, and loricrin. Insets are higher resolution and increased exposure images. Nuclei were stained with DAPI (blue). (B) Immunoblotting analysis of adult control wild-type and Inv-Dsg2 transgenic mouse skin with antibodies against Flag, Dsg1 (antibody 27B2; recognizes mouse Dsg1-{alpha}, Dsg1-beta and Dsg1-{gamma}), Dsg3 (AP904), PG, CK6, CK10, CK14, Lor, Fil and actin (loading control).





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