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Figure 1


Fig. 1. Targeting construct and Dsg2-Flag expression in transgenic mice. (A) Schematic diagram of the mDsg2-Flag transgenic construct inserted at NotI restriction sites downstream of the involucrin promoter in the pH2700-pL2 vector mDsg2, mouse desmoglein 2 cDNA; Flag, nucleotide sequence encoding for the Flag octapeptide; polyA, polyadenylation signal. (B) Immunofluorescent staining of wild-type (upper panels) and Inv-Dsg2 transgenic (lower panels) adult mouse skin with Flag (green, left panels) and Dsg2 MP6 (red, middle panels) antibodies showing expression of Dsg2-Flag in the transgenic, but not control epidermis. Co-staining (merge, right panels) for Dsg2 and Flag in the Inv-Dsg2 transgenic but not control wild-type skin. Nuclei were stained with DAPI (blue). (C) Immunoblot analysis of adult control and transgenic mouse skin extracts using Flag and MP6 antibodies showing Dsg2 and Flag expression in the transgenic but not wild-type skin. Blots were reprobed with actin antibody as a loading control. In all panels: Tg, transgenic; WT, wild type.





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